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LIVING SILICA ® EFFECTS IN COLLAGEN PRODUCTION

IEC Espagne Institute of Spanish Clinical Expertise

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study


At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:


  • Synthesis of Collagen in Human Fibroblasts
  • Cytotoxicity in Human Fibroblasts

Evaluation of Cytotoxicity


The results are presented in the following table:


  Optical density %
  (570nm)  
Negative Test 0.512 ± 0.03  –
Living Silica ® (0.2%) 0.508 ± 0.04 ns
Living Silica ® (0.5%) 0.500 ± 0.06 ns
Living Silica ® (1%) 0.514 ± 0.04 ns
     
 NS: Not Significant    

The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.

Evaluation of the Collagen Index


The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.


  Concentration of hydroxyproline (mg/ml) % increase 
Negative Test 1.64 ± 0.10  –
Living Silica ® 0.2% 1.79 ± 0.13 9
Living Silica ® 0.5% 1.86 ± 0.08 13
Living Silica ® 1% 1.95 ± 0.10 19


The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.

Conclusion


In the experimental conditions adopted for the product Living Silica ® :


  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
  • Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.

Product being investigated:                      Organic Silica (ref: Living Silica ®)

Spanish I.E.C. Code:                                   E080043 130003

Experiment Protocol:                                  No. E080043PE, of February 26, 2008

Authentication

Study No. E080043RD

Study Sponsor

Mr. J.C. Hierro Alonso


Director-General Master’s in microbiology

Silicium Laboratories SL

 I.E.C. Spain

Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.

 

IEC Espagne Institute of Spanish Clinical Expertise

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study

At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:


  • Synthesis of Collagen in Human Fibroblasts
  • Cytotoxicity in Human Fibroblasts

Evaluation of Cytotoxicity

The results are presented in the following table:


  Optical density %
  (570nm)  
Negative Test 0.512 ± 0.03  –
Living Silica ® (0.2%) 0.508 ± 0.04 ns
Living Silica ® (0.5%) 0.500 ± 0.06 ns
Living Silica ® (1%) 0.514 ± 0.04 ns
 NS: Not Significant

The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.

Evaluation of the Collagen Index

The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.


  Concentration of hydroxyproline (mg/ml) % increase 
Negative Test 1.64 ± 0.10  –
Living Silica ® 0.2% 1.79 ± 0.13 9
Living Silica ® 0.5% 1.86 ± 0.08 13
Living Silica ® 1% 1.95 ± 0.10 19

The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.

Conclusion

In the experimental conditions adopted for the product Living Silica ® :


  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
  • Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.

Product being investigated:                      Organic Silica (ref: Living Silica ®)

Spanish I.E.C. Code:                                   E080043 130003

Experiment Protocol:                                  No. E080043PE, of February 26, 2008

Authentication

Study No. E080043RD

Study Sponsor

Mr. J.C. Hierro Alonso

Director-General Master’s in microbiology

Silicium Laboratories SL

 I.E.C. Spain

Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.

 

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