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Living Silica® effects in collagen production


Collagen is the most abundant protein in our body and is essential for maintaining healthy skin, hair, nails, and joints. This article explores the effects of Living Silica on collagen production and how it can benefit overall health.

IEC Espagne Institute of Spanish Clinical Expertise

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study

At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:

  • Synthesis of Collagen in Human Fibroblasts
  • Cytotoxicity in Human Fibroblasts

Evaluation of Cytotoxicity

The results are presented in the following table:



Optical density %
Negative Test0.512 ± 0.03 –
Living Silica ® (0.2%)0.508 ± 0.04ns
Living Silica ® (0.5%)0.500 ± 0.06ns
Living Silica ® (1%)0.514 ± 0.04ns

 NS: Not Significant

The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.

Evaluation of the Collagen Index

The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.

 Concentration of hydroxyproline (mg/ml)% increase 
Negative Test1.64 ± 0.10 –
Living Silica ® 0.2%1.79 ± 0.139
Living Silica ® 0.5%1.86 ± 0.0813
Living Silica ® 1%1.95 ± 0.1019

The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.


In the experimental conditions adopted for the product Living Silica ® :

  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
  • Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.

Product being investigated: Organic Silica (ref: Living Silica ®)

Spanish I.E.C. Code: E080043 130003

Experiment Protocol: No. E080043PE, of February 26, 2008


  • Study No. E080043RD
  • Study Sponsor
  • Mr. J.C. Hierro Alonso
  • Director-General Master’s in microbiology
  • Silicium Laboratories SL
  •  I.E.C. Spain
  • Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.

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