Learn how organic silica can enhance bone health by increasing collagen synthesis and calcium deposition in osteoblasts.
Evaluation of Living Silica® on Bone Health fibroblasts, Osteoblasts and MG-63 Cell Line.
Performed at the Technology Centre for Nutrition and Health Survey.
How organic silica can help your bone health?
Several authors have emphasized the role of silicon in health, being the most prominent its effect on the integrity of the nails, hair and skin as well as in the synthesis of collagen and bone mineralization. It was observed that silica deficiency has adverse effects such as bone deformities, joint malfunction, reducing the mass of cartilage and rupture of the mineral balance in femur and vertebrae.
One of the most suitable ways to determine possible effects of a bioactive compound or supplement is the use of in vitro systems on certain cell types, thus avoiding hormonal or endocrine interactions. The model chosen for this study was the MG-63 cell-line, which has a fibroblast phenotype that can be differentiated and give place to different populations of mature cells, such as osteoblasts.
The aim of this study is to evaluate the effects of Loic Le Ribault’s, Living Silica®, silica on the production of collagen in the undifferentiated (fibroblasts) MG-63 cell line and the effects on differentiation and mineralization of osteoblasts.
Results of our Research:
Effects of Living Silica® on collagen production in the short term:
We evaluated the effects of treatment with 200, 1000, and 2000 mg/liter of silicon on collagen production. The results indicated its induction 24 hours after the initiation of treatment compared to untreated cells.
Effects of Living Silica® on collagen production in the long term:
The same process performed over 16 days resulted in a net increase in collagen production, showing a dose-response action. The ratio of collagenous and non-collagenous proteins resulted in a significant 12% increase for all doses tested, indicating that the three treatments induce collagen synthesis.
Effects of Living Silica® on calcium deposits in osteoblasts during differentiation
We studied whether treatments with Living Silica® affected the calcification of MG-63 osteoblasts. The results showed an increase in calcium deposition following a dose-response action, which was statistically significant compared to untreated cells. There was a 40% increase in calcification with a dosage of 2000 mg/liter.
Effects of Living Silica® on collagen synthesis in osteoblasts during the process of differentiation:
The results showed that treatment with the concentrated 2000 mg/liter product increased collagen levels in differentiated osteoblasts.
Living Silica® Effects on osteoblast differentiation:
With the 2000 mg/liter product, a significant increase in the activity of alkaline phosphatase was observed.
The results of the study show that collagen synthesis is enhanced by treatment with Loïc Le Ribault’s Living Silica®, silica cells in the form of fibroblasts, indicating possible effects on bone mass since it consists largely of this protein. The results suggest beneficial effects, with the highest dose increasing the concentration of collagen. Secondly, treatment with Loïc Le Ribault’s Living Silica®, silica in osteoblasts in the process of differentiation results in an increase in calcification compared to untreated cells, as indicated by increased calcium deposition. The study suggests that Living Silica® is capable of increasing mineralization of cultured osteoblasts.
There could be two reasons for the observed increase in calcium deposit located in the osteoblasts. Firstly, the greater differentiation of fibroblasts induced by treatment results in an increased number of osteoblasts, thereby proportionally increasing calcification. Secondly, an increase in calcium in the metabolism or cellular processes involved in the deposition of calcium would explain increased calcification.
In mature osteoblasts, treatment with Loïc Le Ribault’s Living Silica®, silica results in a significant increase in alkaline phosphatase activity. Since this activity depends on the concentration of collagen in the extracellular matrix and is responsible for calcification, the increased collagen synthesis induced by treatment using Living Silica® was responsible for the increased activity of alkaline phosphatase. It should be noted that the determination of collagen and alkaline phosphatase was achieved in two different experiments.
The results of this study indicate that treatment with Loïc Le Ribault’s Living Silica®, silica increases collagen synthesis and reinforces the extracellular matrix in MG-63 cells. This effect potentiates calcium deposition by osteoblasts, increasing calcification of the extracellular matrix. If these results were applicable to humans, the use of Living Silica® would be beneficial in situations that require reinforcement of the bone matrix in the context of an adequate intake of necessary components required to induce differentiation from fibroblasts to osteoblasts or osteogenesis.
Centro Tecnológico de Nutrición y Salud
Dr. José María del Bas, Dr. Luis Arola y Dr. Francesc Puiggròs