IEC Espagne Institute of Spanish Clinical Expertise 

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study

At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of the Loïc Le Ribault’s G5 organic silica in the following parameters:

  • Synthesis of Collagen in Human Fibroblasts
  • Cytotoxicity in Human Fibroblasts

What is collagen?

Collagen is the most abundant protein and is naturally produced by the body. The production of this substance diminishes as people grow older. It is significant in making certain parts of the body and is also an important factor in joint health, bonescartilagetendonsligamentsmuscle tissuecorneasblood vesselsfasciagut, intervertebral discsteeth & gums. It is also important on the moisture, smoothness, elasticity, and flexibility of the skin, hair & nails.

Evaluation of Cytotoxicity

The results are presented in the following table:

  Optical density %
Negative Test 0.512 ± 0.03  –
Organic Silica (0.2%) 0.508 ± 0.04 ns
Organic Silica (0.5%) 0.500 ± 0.06 ns
Organic Silica (1%) 0.514 ± 0.04 ns
 NS: Not Significant    

The results show that the Loïc Le Ribault’s G5 organic silica does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.

Evaluation of the Collagen Index

The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.

  Concentration of hydroxyproline (mg/ml) % increase 
Negative Test 1.64 ± 0.10  –
Organic Silica G5 0.2% 1.79 ± 0.13 9
Organic Silica G5 0.5% 1.86 ± 0.08 13
Organic Silica G5 1% 1.95 ± 0.10 19

The results show that Loïc Le Ribault’s G5 Organic Silica significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.


In the experimental conditions adopted for the product Organic Silica G5:

  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.  
  • Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Organic Silica G5 product induces a significant increase in the index in cultured human fibroblasts.

Product being investigated:                      Organic Silica (ref: Silicium G5)

Spanish I.E.C. Code:                                   E080043 130003 

Experiment Protocol:                                  No. E080043PE, of February 26, 2008


Study No. E080043RD

Study Sponsor            

Mr. J.C. Hierro Alonso                                                                        

Director-General Master’s in microbiology        

Silicium Espana                                                                                

 I.E.C. Spain

Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.

Comments are closed.

Leave A Comment

Comments are closed.

Cart Item Removed. Undo
  • No products in the cart.
Lost your password? Please enter your username or email address. You will receive a link to create a new password via email.
We do not share your personal details with anyone.