IEC Espagne Institute of Spanish Clinical Expertise 

  Silicium Laboratories LLC sponsored an independent study in 2012 consisting of examining the effects of consuming Living Silica ® Collagen Booster liquid, and topically applying Living Silica ® Collagen Booster gel over a six-week period in a sample group of males and females aged 50 years and older who were experiencing various degrees of osteoarthritis. The results are shown below in the chart below.

Study of product effects on the production of collagen neo-synthesis in human fibroblasts.

Report:  In Vitro Clinical Study

At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:

  • Synthesis of Collagen in Human Fibroblasts
  • Cytotoxicity in Human Fibroblasts

What is collagen?

Collagen is the most abundant protein and is naturally produced by the body. The production of this substance diminishes as people grow older. It is significant in making certain parts of the body and is also an important factor in joint health, bonescartilagetendonsligamentsmuscle tissuecorneasblood vesselsfasciagut, intervertebral discsteeth & gums. It is also important on the moisture, smoothness, elasticity, and flexibility of the skin, hair & nails.

Evaluation of Cytotoxicity

The results are presented in the following table:

 Optical density%
Negative Test0.512 ± 0.03 –
Living Silica ® (0.2%)0.508 ± 0.04ns
Living Silica ® (0.5%)0.500 ± 0.06ns
Living Silica ® (1%)0.514 ± 0.04ns
 NS: Not Significant  

The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.

Evaluation of the Collagen Index

The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.

 Concentration of hydroxyproline (mg/ml)% increase 
Negative Test1.64 ± 0.10 –
Living Silica ® 0.2%1.79 ± 0.139
Living Silica ® 0.5%1.86 ± 0.0813
Living Silica ® 1%1.95 ± 0.1019


The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.


In the experimental conditions adopted for the product Living Silica ® :

  • Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.  
  • Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)

In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.

Product being investigated:                      Organic Silica (ref: Living Silica ®)

Spanish I.E.C. Code:                                   E080043 130003 

Experiment Protocol:                                  No. E080043PE, of February 26, 2008


Study No. E080043RD

Study Sponsor            

Mr. J.C. Hierro Alonso                                                                        


Director-General Master’s in microbiology        

Silicium Laboratories SL

 I.E.C. Spain

Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.

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